The proliferation of NIH/3T3 cells treated with increasing concentrations of mFGF basic was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.
The purity of recombinant mFGF basic was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mFGF basic and staining overnight with Coomassie Blue.
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with mFGF basic for 10 min, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10mM DTT and 20 μg BSA per 1 μg mFGF basic. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10mM DTT.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mFGF basic. All lots are greater than 98% pure.
Recombinant mFGF basic does not have a Met on the amino terminus and has a calculated MW of 16,354. DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides. The expected amino terminal ALPED of recombinant mFGF basic was verified by amino acid sequencing.
Recombinant mouse FGF basic (mFGF basic) Ala11-Ser154 (Accession #NP_032032) was produced in E. coli at Cell Signaling Technology.
FGF basic (FGF2) is producted in both embryonic and adult cell types, and contributes to the pathogenesis of various diseases, including cancer and atherosclerosis (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).
Explore pathways + proteins related to this product.
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.