Interested in promotions? | Click here >>
50944
Mouse IL-17A Recombinant Protein
Cytokines
Growth Factors and Cytokines

Mouse IL-17A Recombinant Protein #50944

Reviews ()
Citations (0)
Mouse IL-17A Recombinant Protein: Image 1
Serial dilutions of Mouse IL-17A Recombinant Protein were added to NIH/3T3 cells. Secretion of IL-6 was measured and the linear portion of the curve was used to calculate the ED50.
Mouse IL-17A Recombinant Protein: Image 2
The purity of Mouse IL-17A Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mIL-17A and staining with Coomassie Blue. mIL-17A is a homodimer with a predicted total molecular weight (MW) of 30.2 kDa with each subunit equaling 15.1 kDa.

Storage

Mouse IL-17A Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

MW (kDa) 30.2
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-17A.
Activity The bioactivity of recombinant mIL-17A was determined by measuring the secretion of IL-6 by NIH/3T3 cells. The ED50 of each lot is less than or equal to 5 ng/ml.

Source / Purification

Recombinant mouse IL-17A was expressed in E. coli and is supplied in a lyophilized form

Background

Interleukin-17A (IL-17A) is a cystine-linked homodimeric pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates production of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to IL-17RA (5). IL-17A binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6). Phosphorylation of some Jaks and Stats has been observed.
  1. Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.
  2. Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.
  3. Dubin, P.J. and Kolls, J.K. (2008) Immunol Rev 226, 160-71.
  4. Zrioual, S. et al. (2009) J Immunol 182, 3112-20.
  5. Wright, J.F. et al. (2008) J Immunol 181, 2799-805.
  6. Rahman, M.S. et al. (2006) J Immunol 177, 4064-71.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 50944S
Product # Size Price
50944S
25 µg $ 247