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5224
Mouse Interleukin-22 (mIL-22)
Cytokines
Growth Factors and Cytokines

Mouse Interleukin-22 (mIL-22) #5224

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Mouse Interleukin-22 (mIL-22): Image 1

The production of IL-10 by COLO 205 cells cultured with increasing concentrations of mIL-22 was assessed. Media from cells incubated with mIL-22 for 48 hours was collected and assayed for IL-10 by ELISA and the OD450-OD650 was determined.

Mouse Interleukin-22 (mIL-22): Image 2

The purity of recombinant mIL-22 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-22 and staining overnight with Coomassie Blue.

Mouse Interleukin-22 (mIL-22): Image 3

Western blot analysis of extracts from HT-29 cells, untreated or treated with mIL-22 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-22. All lots are greater than 98% pure.

Molecular Formula

Recombinant mIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,640. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal LPVNT of recombinant mIL-22 was verified by amino acid sequencing.

Source / Purification

Recombinant mouse IL-22 (mIL-22) Leu34-Val179 (Accession #NP_058667) was expressed in human 293 cells at Cell Signaling Technology.

Background

IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). Expression of IL-22 in combination with a select group of cytokines defines a subset of Th cells(4). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (5). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (6).

  1. Ouyang, W. et al. (2008) Immunity 28, 454-67.
  2. Dumoutier, L. et al. (2000) Proc Natl Acad Sci U S A 97, 10144-9.
  3. Takatori, H. et al. (2008) Mod Rheumatol 18, 533-41.
  4. Eyerich, S. et al. (2009) J Clin Invest 119, 3573-85.
  5. Nagalakshmi, M.L. et al. (2004) Int Immunopharmacol 4, 679-91.
  6. Aujla, S.J. et al. (2008) Nat Med 14, 275-81.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 5224SC
Product # Size Price
5224SC
10 µg  (With Carrier) $ 221
5224SF
10 µg  (Carrier Free) $ 221