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8923
Mouse Interleukin-3 (mIL-3)

Mouse Interleukin-3 (mIL-3) #8923

Coomassie Gel Image 1

The purity of recombinant mIL-3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-3 and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The proliferation of BaF3 cells treated with increasing concentrations of mIL-3 was assessed. After 48 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

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Bioactivity Image 3

The proliferation of MC/9 cells treated with increasing concentrations of mIL-3 was assessed. After 72 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

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Western Blotting Image 4

Western blot analysis of extracts from BaF3 cells, untreated or treated with mIL-3 for 10 minutes, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (upper) and Stat5 (3H7) Rabbit mAb #9358 (lower).

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Recombinant mouse interleukin-3 (mIL-3) Ala27-Cys166 (Accession # NM_010556) was produced in E. coli at Cell Signaling Technology.

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-3. All lots are greater than 98% pure.

Molecular Formula:

Recombinant mIL-3 does not have a Met on the amino terminus and has a calculated MW of 15,674. DTT-reduced protein migrates as a 16 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal ASISG of recombinant mIL-3 was verified by amino acid sequencing.

Bioactivity:

The bioactivity of recombinant mIL-3 was determined in MC/9 and BaF3 cell proliferation assays. The ED50 of each lot is between 2-50 pg/ml in MC/9 cells and 20-90 pg/mL in BaF3 cells.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg mIL-3.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-3 is produced by T cells, mast cells and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3,4,5).

  1. Reddy, E.P. et al. (2000) Oncogene 19, 2532-47.
  2. Hara, T. and Miyajima, A. (1992) EMBO J 11, 1875-84.
  3. Asquith, K.L. et al. (2008) J Immunol 180, 1199-206.
  4. Schroeder, J.T. et al. (2009) J Immunol 182, 2432-8.
  5. Munitz, A. et al. (2006) J Immunol 177, 77-83.
Entrez-Gene Id
16187
Swiss-Prot Acc.
P01586
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 8923SC

Product Number Size Price
8923SC 10 µg (With Carrier) $204.00.0
8923SF 10 µg (Carrier Free) $204.00.0
Quantity Subtotal
$0.00