With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
|Purity||>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-3. All lots are greater than 98% pure.|
|Endotoxin||Less than 0.01 ng endotoxin/1 μg mIL-3.|
|Activity||The bioactivity of recombinant mIL-3 was determined in MC/9 and BaF3 cell proliferation assays. The ED50 of each lot is between 2-50 pg/ml in MC/9 cells and 20-90 pg/mL in BaF3 cells.|
|Molecular Formula||Recombinant mIL-3 does not have a Met on the amino terminus and has a calculated MW of 15,674. DTT-reduced protein migrates as a 16 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal ASISG of recombinant mIL-3 was verified by amino acid sequencing.|
Recombinant mouse interleukin-3 (mIL-3) Ala27-Cys166 (Accession # NM_010556) was produced in E. coli at Cell Signaling Technology.
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