View Featured Offers >>
2169
Tumor Necrosis Factor-α
Cytokines & Growth Factors
Growth Factors and Cytokines

Tumor Necrosis Factor-α #2169

Citations (0)

We recommend the following alternatives

# Product Name Applications Reactivity
Western blot analysis of HeLa cells stimulated with rhTNF-α (10 ng/ml for 5 minutes), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 (upper) and IKKα Antibody #2682 (lower).

Product Usage Information

CST recommends using 10-100 ng/ml of rhTNF-alpha for stimulation of TNF-alpha signaling.

Storage

rhTNF-α is supplied as a 100 μg/ml solution. It should be stored at -80°C. Aliquot the reagent upon receipt and avoid repeated freeze-thaw cycles.

Product Description

Recombinant human TNF-alpha (rhTNF-alpha) protein is expressed in E. coli, purified, and stored in PBS buffer containing 0.1% BSA. It is supplied as a 100 μg/ml solution and should be aliquoted and stored at -80ºC to avoid repeated freeze/thaw cycles.
MW (kDa) 17.3
Purity >95%
Activity 100 µg/ml, 5 x 107 IU/mg

Source / Purification

Human Recombinant Protein expressed in E. coli.

Background

TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).

Pathways

Explore pathways related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
Inquiry Info.# 2169