FastScan^™ LbCpf1 (Strain ND2006)/Cas12a ELISA Kit

1 Kit

(96 assays)

Species Cross Reactivity:
All

UniProt ID:
#A0A182DWE3

Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

[email protected]

cellsignal.com

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Color
FastScan™ ELISA Microwell Strip Plate, 96 Well	53257	96 tests
LbCpf1 (Strain ND2006)/Cas12a Mouse Capture mAb	37601	1 ea	Green (Lyophilized)
LbCpf1 (Strain ND2006)/Cas12a Rabbit HRP-linked mAb	64873	1 ea	Red (Lyophilized)
FastScan™ ELISA Capture Antibody Diluent	16076	3 ml	Green
FastScan™ ELISA HRP Antibody Diluent	28120	3 ml
TMB Substrate	7004	11 ml
STOP Solution	7002	11 ml
Sealing Tape	54503	1 ea
ELISA Wash Buffer (20X)	9801	25 ml
FastScan™ ELISA Cell Extraction Buffer (5X)	69905	10 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X)	25243	1 ml
FastScan™ ELISA Kit #88197 Positive Control Type 2	70692	1 ea

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.

Description

The FastScan™ LbCpf1 (Strain ND2006)/Cas12a ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of LbCpf1 (Strain ND2006)/Cas12a protein. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with LbCpf1 (Strain ND2006)/Cas12a protein in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of LbCpf1 (Strain ND2006)/Cas12a protein.

*Antibodies in this kit are custom formulations specific to kit.

IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.

Specificity/Sensitivity

The FastScan™ LbCpf1 (Strain ND2006)/Cas12a ELISA Kit detects endogenous levels of LbCpf1 (Strain ND2006)/Cas12a protein, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).~LbCpf1 (Strain ND2006)/Cas12a is a Cpf1/Cas12a enzyme derived from Lachnospiraceae bacterium ND2006 (2,4).

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com