| Product Includes | Product # | Quantity | Color |
|---|---|---|---|
| FastScan™ ELISA Microwell Strip Plate, 96 Well | 53257 | 96 tests | |
| Neurogranin Rabbit Capture mAb | 33414 | 1 ea | Green (Lyophilized) |
| Neurogranin Rabbit HRP-linked mAb | 58057 | 1 ea | Red (Lyophilized) |
| FastScan™ ELISA Capture Antibody Diluent | 16076 | 3 ml | Green |
| FastScan™ ELISA HRP Antibody Diluent | 28120 | 3 ml | |
| TMB Substrate | 7004 | 11 ml | |
| STOP Solution | 7002 | 11 ml | |
| Sealing Tape | 54503 | 1 ea | |
| ELISA Wash Buffer (20X) | 9801 | 25 ml | |
| FastScan™ ELISA Cell Extraction Buffer (5X) | 69905 | 10 ml | |
| FastScan™ ELISA Cell Extraction Enhancer Solution (50X) | 25243 | 1 ml | |
| FastScan™ ELISA Kit #99828 Positive Control Type 1 | 96367 | 1 ea |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The FastScan™ Total Neurogranin ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of neurogranin. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with neurogranin in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of neurogranin.
*Antibodies in kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
Neurogranin is a small neuronal protein that binds to calmodulin in the absence of Ca2+ (1,2). Calmodulin binds Ca2+ and acts as a Ca2+ sensor to drive Ca2+-dependent signal transduction pathways by modulating its interaction with various kinases and phosphatases (3). In the postsynaptic compartment of the neuron, Ca2+ is carefully regulated and is a critical regulator of synaptic function and plasticity (4). Neurogranin is primarily expressed in the brain and is enriched in somato-dendritic compartments of projection neurons in various regions of the brain (5). Postsynaptically-enriched neurogranin likely influences Ca2+ or Ca2+/calmodulin-dependent neuronal functions, including synaptic plasticity, by binding to and releasing calmodulin in a Ca2+-dependent manner (6-9). Genetic variants in the gene encoding neurogranin, NRGN, are linked to several neuropsychiatric diseases, including schizophrenia (10). Interestingly, increase in cerebrospinal fluid neurogranin is correlated with neurodegenerative disease progression, which suggests that neurogranin could act as a biomarker for diseases like Alzheimer’s disease (11).
- Baudier, J. et al. (1991) J Biol Chem 266, 229-37.
- Gispen, W.H. et al. (1991) Mol Neurobiol 5, 61-85.
- Chin, D. and Means, A.R. (2000) Trends Cell Biol 10, 322-8.
- Blackstone, C. and Sheng, M. (2002) Front Biosci 7, d872-85.
- Gerendasy, D.D. and Sutcliffe, J.G. (1997) Mol Neurobiol 15, 131-63.
- Huang, K.P. et al. (2004) J Neurosci 24, 10660-9.
- Pak, J.H. et al. (2000) Proc Natl Acad Sci U S A 97, 11232-7.
- Zhong, L. et al. (2009) EMBO J 28, 3027-39.
- Jones, K.J. et al. (2018) Proc Natl Acad Sci U S A 115, E5805-E5814.
- Jin, L. et al. (2019) Medicine (Baltimore) 98, e18518.
- Willemse, E.A.J. et al. (2018) Clin Chem 64, 927-37.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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