Revision 6

#13125

Store at +4C

PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit

1 Kit

(96 assays)

Species Cross Reactivity:
H

UniProt ID:
#Q15303

Entrez-Gene Id:
#2066

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Color Storage Temp
HER4/ErbB4 Rabbit mAb Coated Microwells9746796 tests+4C
Phospho Tyrosine Mouse Detection mAb129821 eaGreen (Lyophilized)+4C
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated)133041 eaRed (Lyophilized)+4C
Detection Antibody Diluent1333911 mlGreen+4C
HRP Diluent1351511 mlRed+4C
TMB Substrate700411 ml+4C
STOP Solution700211 ml+4C
Sealing Tape545032 ea+4C
ELISA Wash Buffer (20X)980125 ml+4C
ELISA Sample Diluent1108325 mlBlue+4C
Cell Lysis Buffer (10X)980315 ml-20C

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.

Description

The PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-HER4/ErbB4 (panTyr) protein. A HER4/ErbB4 rabbit mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-HER4/ErbB4 proteins are captured by the coated antibody. Following extensive washing, a phospho-tyrosine mouse antibody is added to detect captured tyrosine-phosphorylated HER4/ErbB4 proteins. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of HER4/ErbB4 phosphorylated on tyrosine residues.

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit #13125 detects endogenous levels of tyrosine-phosphorylated HER4/ErbB4 protein in human cells, as shown in Figure 1. This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Figure 1: Treatment of T-47D cells with Human Neuregulin-1 (hNRG-1) stimulates tyrosine phosphorylation of HER4/ErbB4 protein, as detected by PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit #13125, but does not affect the level of total HER4/ErbB4 detected by PathScan® Total HER4/ErbB4 Sandwich ELISA Kit #13010. The absorbance readings at 450 nm are shown in the top figure while corresponding western blots using HER4/ErbB4 (111B2) Rabbit mAb #4795 (left panel) and Phospho-HER4/ErbB4 (Tyr984) Antibody #3790 (right panel) are shown in the bottom figure.
ELISA Image 1: PathScan<sup>®</sup> Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit
Figure 2: The relationship between protein concentration of lysates from untreated and hNRG-1-treated T-47D cells and the absorbance at 450 nm as detected by the PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit is shown. Starved T-47D cells (85% confluence) were treated with Human Neuregulin-1 (hNRG-1) (100 ng/ml) for 2-5 min at 37°C and then lysed.
ELISA Image 2: PathScan<sup>®</sup> Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.