CST's PathScan® Phospho-p44 MAPK (Thr202/Tyr204) Sandwich ELISA Antibody Pair is offered as an alternative to our PathScan® Phospho-p44 MAPK (Thr202/Tyr204) Sandwich ELISA Kit, #7315. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. p44 MAPK Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by a Phospho-p44/42 MAPK (Thr202/Tyr204) Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of phospho-p44/42 MAPK (Thr202/Tyr204) protein.
Antibodies in kit are custom formulations specific to kit.
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