Interested in promotions? | Click here >>
7193
PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit
ELISA Kits
ELISA Kit

PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit #7193

Citations (0)
PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit: Image 1

Figure 1: HeLa cells synchronized with nocodazole accumulate phosphorylation of survivin at Thr34, as detected by PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit #7193. The accumulated total survivin protein can be detected by PathScan® Total Survivin Sandwich ELISA Kit #7169. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Survivin Mouse mAb #2802 (left panel) or Phospho-Survivin (Thr34) Rabbit Antibody (right panel) are shown in the bottom figure.

PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit: Image 2

Figure 2: The relationship between protein concentration of lysates from untreated and nocodazole-treated HeLa cells and the absorbance at 450 nm is shown. After starvation, HeLa cells (85% confluence) were synchronized with nocodazole (50 ng/ml) for 20 hr at 37ºC and then lysed. Added 1X Phosphatase Inhibitor Cocktail (100X) #5870 to lysis buffer in addition to the 1mM phenyl-methylsulfonyl fluoride (PMSF)

Inquiry Info.# 7193

Important Ordering Details

Custom Ordering Details: When ordering five or more kits, please contact us for processing time and pricing at [email protected].

Supporting Data

REACTIVITY

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

The PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of survivin protein phosphorylated at Thr34. A phospho-survivin (Thr34) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-survivin protein is captured by the coated antibody. Following extensive washing, a survivin mouse antibody is added to detect the captured phospho-survivin protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of survivin phosphorylated at Thr34.

Antibodies in kit are custom formulations specific to kit.

MW (kDa) 16

Specificity / Sensitivity

CST's PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit #7193 detects endogenous levels of survivin protein phosphorylated at Thr34 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).

  1. Reed, J.C. and Reed, S.I. (1999) Nat Cell Biol 1, E199-200.
  2. Li, F. et al. (1998) Nature 396, 580-584.
  3. Li, F. et al. (1999) Nat Cell Biol 1, 461-6.
  4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
  5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
  6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.