| Product Includes | Product # | Quantity | Color | Storage Temp |
|---|---|---|---|---|
| GDF15/MIC1 Rabbit mAb Coated Microwells | 90822 | 96 tests |
|
+4C |
| GDF15/MIC1 Rabbit Detection mAb | 98486 | 1 ea |
|
+4C |
| HRP Diluent | 13515 | 5.5 ml |
|
+4C |
| TMB Substrate | 7004 | 11 ml |
|
+4C |
| STOP Solution | 7002 | 11 ml |
|
+4C |
| Sealing Tape | 54503 | 2 ea |
|
+4C |
| ELISA Wash Buffer (20X) | 9801 | 25 ml |
|
+4C |
| Cell Lysis Buffer (10X) | 9803 | 15 ml |
|
-20C |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The rapid protocol (RP) PathScan® RP GDF15/MIC1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of GDF15/MIC1 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with GDF15/MIC1 protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of GDF15/MIC1 protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-β (TGF-β) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site, generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenesis, and metastasis, and may also contribute to weight loss during cancer (10,11).
- Strelau, J. et al. (2000) J Neurosci 20, 8597-603.
- Yokoyama-Kobayashi, M. et al. (1997) J Biochem 122, 622-6.
- Hromas, R. et al. (1997) Biochim Biophys Acta 1354, 40-4.
- Paralkar, V.M. et al. (1998) J Biol Chem 273, 13760-7.
- Baek, S.J. et al. (2001) J Biol Chem 276, 33384-92.
- Bootcov, M.R. et al. (1997) Proc Natl Acad Sci USA 94, 11514-9.
- Strelau, J. et al. (2000) J Neural Transm Suppl, 273-6.
- Kannan, K. et al. (2000) FEBS Lett 470, 77-82.
- Yang, H. et al. (2003) Mol Cancer Ther 2, 1023-9.
- Johnen, H. et al. (2007) Nat Med 13, 1333-40.
- Bauskin, A.R. et al. (2006) Cancer Res 66, 4983-6.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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