PathScan^® RP Stathmin-2 Sandwich ELISA Kit

1 Kit

(96 assays)

Species Cross Reactivity:
M R

UniProt ID:
#Q93045

Entrez-Gene Id:
#11075

Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

[email protected]

cellsignal.com

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Color	Storage Temp
Stathmin-2 Rabbit mAb Coated Microwells	52792	96 tests		+4C
Stathmin-2 Rabbit Detection mAb	96235	1 ea	Red (Lyophilized)	+4C
HRP Diluent	13515	5.5 ml	Red	+4C
TMB Substrate	7004	11 ml		+4C
STOP Solution	7002	11 ml		+4C
Sealing Tape	54503	2 ea		+4C
ELISA Wash Buffer (20X)	9801	25 ml		+4C
Cell Lysis Buffer (10X)	9803	15 ml		-20C

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.

Description

The rapid protocol (RP) PathScan^® RP Stathmin-2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stathmin-2 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Stathmin-2 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Stathmin-2. Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

The PathScan^® RP Stathmin-2 Sandwich ELISA Kit detects endogenous levels of Stathmin-2 protein. This kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Stathmin is a family of microtubule-destabilizing phosphoproteins that play an important role in regulation of the microtubule cytoskeleton (1). The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules, regulating cellular processes, such as cell cycle and neuronal axon development (2). Stathmin family members include stathmin, stathmin-2, stathmin-3, and stathmin-4. Interestingly, stathmin-2 protein levels correlate with decreased TDP43 expression, an mRNA-binding protein that mislocalizes and aggregates in patients with the motor neuron disease, amyotrophic lateral sclerosis (ALS) (3). Interestingly, TDP43 directly regulates stathmin-2 expression by regulating STMN2 RNA splicing, suggesting that altered stathmin-2 expression might serve as a biomarker for disease progression or a therapeutic target.

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com