Interested in promotions? | Click here >>
PathScan® Total IRS-2 Sandwich ELISA Kit

PathScan® Total IRS-2 Sandwich ELISA Kit #7884

Citations (0)
PathScan® Total IRS-2 Sandwich ELISA Kit: Image 1

Figure 1. Treatment of CHO (IR/4PS) cells with insulin stimulates phosphorylation of IRS-2 at tyrosine residues as detected by PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA #7861, but does not affect the level of total IRS-2 protein detected by PathScan® Total IRS-2 Sandwich ELISA #7884. CHO cells stably transfected with the insulin receptor and IRS-2 (CHO (IR/4PS) cells) were starved overnight and treated with 100 nM insulin for 2 minutes at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots are shown in the bottom figure. In order to detect tyrosine phosphorylated IRS-2, total IRS-2 was immunoprecipitated using microwells coated with an IRS-2-specific antibody. The wells were washed and the protein solubilized with SDS loading buffer. The captured protein was then separated by gel electrophoresis and probed with either IRS-2 Antibody #4502 (left panel) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (right panel).

PathScan® Total IRS-2 Sandwich ELISA Kit: Image 2

Figure 2. The relationship between the protein concentration of the lysate from untransfected CHO cells or CHO cells stably transfected with the insulin receptor + IRS-2 and the absorbance at 450 nm is shown.

To Purchase # 7884

Important Ordering Details

Custom Ordering Details: When ordering five or more kits, please contact us for processing time and pricing at [email protected].

Supporting Data


Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

The PathScan® Total IRS-2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total IRS-2 (also called 4PS). An IRS-2 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, IRS-2 (both phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a biotinylated IRS-2 rabbit antibody is added to detect the captured IRS-2. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate (TMB) is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2.

Antibodies in kit are custom formulations specific to kit.

Specificity / Sensitivity

CST's PathScan® Total IRS-2 Sandwich ELISA Kit #7884 detects endogenous levels of IRS-2. As shown in Figure 1, using the PathScan® Total IRS-2 Sandwich ELISA Kit #7884, a high level of IRS-2 is detected in transfected CHO cells. The total levels of IRS-2 (phospho and nonphospho) remain unchanged after insulin treatment as shown by western analysis. This kit also detects endogenous levels of IRS-2 expressed by 3T3-L1 adipocytes (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

  1. Sun, X.J. et al. (1991) Nature 352, 73-77.
  2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
  3. Withers, D.J. et al. (1998) Nature 391, 900-904.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.