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PhosphoSitePlus® Resource

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REACTIVITY
Product Includes Volume Solution Color
Ret Rabbit mAb Coated Microwells 96 tests
Sealing Tape 2 sheets

Product Description

The PathScan® Total Ret Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total Ret protein. A Ret rabbit mAb has been coated on the microwells. After incubation with cell lysates, Ret protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Ret mouse mAb is added to detect captured Ret protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total Ret protein.


Specificity / Sensitivity

PathScan® Total Ret Sandwich ELISA Kit #7032 detects endogenous levels of total Ret protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5).


1.  Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25.

2.  Takahashi, M. et al. (1989) Oncogene 4, 805-6.

3.  Manié, S. et al. (2001) Trends Genet 17, 580-9.

4.  Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.

5.  Iwashita, T. et al. (1999) Oncogene 18, 3919-22.


Entrez-Gene Id 5979
Swiss-Prot Acc. P07949


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7032
PathScan® Total Ret Sandwich ELISA Kit