Boil for 3 minutes prior to use. Load 10 μl of phosphorylated and nonphosphorylated 4E-BP1 Control Cell Extracts per lane.
Store at -20°C. Supplied in SDS Sample Buffer: 62.5 mM Tris- HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red.
|MW (kDa)||15 to 20|
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