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9213
p38 MAPK Control Cell Extracts
Experimental Controls
Cell Extract Kit

p38 MAPK Control Cell Extracts #9213

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  1. WB
Western blot analysis of p38 MAPK Control Cell extracts using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb

#4511 (upper) and p38 MAPK Antibody #9212 (lower).

Product Usage Information

Boil for 3 minutes prior to use. Load 15 µl of phosphorylated and nonphosphorylated p38 MAP Kinase Control Cell Extracts per lane.

Storage

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

Product Description

Nonphosphorylated p38 MAPK Control Cell Extracts: Total extracts from C-6 glioma cells to serve as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated p38 MAPK Control Cell Extracts: Total extracts from C-6 glioma cells treated with Anisomycin #2222 at 25 ug/ml for 30 minutes to serve as a positive control. Supplied in SDS Sample Buffer.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 9213
Cat. # Size Qty. Price
9213S
150 µl  (10 western blots)