News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
CST Antibody Performance Guarantee
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
Rat C6 Apoptosis Cell Lysates (staurosporine) #9543
This product is discontinued
Gallery: Rat C6 Apoptosis Cell Lysates (staurosporine) #9543
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
Description: Total cell lysates from Rat glioma C6 cells, untreated or treated with 1 mM staurosporine to activate apoptotic cascades and induce proteolytic cleavage of various apoptosis-related proteins including caspases and PARP. This lysate pair is produced as a molecular weight control for western blotting. Boil lysates for 2 minutes in the original tube, then load 10-15 ml per mini-gel lane.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.