Boil for 3 minutes prior to use. Load 20 µl of phosphorylated and nonphosphorylated Akt extracts per lane.
Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red.
Store at –20°C, or at –80°C for long-term storage.
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