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1050
Cleaved Caspase-3 (Asp175) Blocking Peptide

Cleaved Caspase-3 (Asp175) Blocking Peptide #1050

APPLICATIONS

Western Blotting

Western blot analysis of cell extracts from Jurkat cells treated with 0.25 mg/ml cytochrome c, using Cleaved Caspase-3 (Asp175) Antibody #9661 without (left) and with (right) preincubation with cleavage-peptide.

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IHC-P (paraffin)

Immunohistochemical staining of paraffin-embedded human tonsil, using Cleaved Caspase-3 (Asp175) Antibody #9661 preincubated with irrelevant control peptide (left) and with Cleaved Caspase-3 (Asp175) Blocking Peptide (right).

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This peptide is used to block Cleaved Caspase-3 (Asp175) Antibody #9661 and #9664 reactivity.

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cleaved Caspase-3 (Asp175) Antibody #9661 by immunohistochemistry and Western Blotting.

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry and Western blot protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room

temperature for 30 minutes before allowing to react with the blot.

Storage:

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5% glycerol and 1% DMSO. Store at –20°C.

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.
Entrez-Gene Id
836
Swiss-Prot Acc.
P42574
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 1050S

Product Number Size Price
1050S 100 µg $81.00.0
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