Buy 3 and get the 4th FREE!* | Learn More >>
1033
Cytochrome c Blocking Peptide
Experimental Controls

Cytochrome c Blocking Peptide #1033

Reviews ()
Citations (0)

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Storage:

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Product Description

This peptide is used to block Cytochrome c (136F3) Rabbit mAb #4280 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cytochrome c (136F3) Rabbit mAb #4280 by dot blot.

Background

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).

  1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
  2. Li, P. et al. (1997) Cell 91, 479-89.
  3. Liu, X. et al. (1996) Cell 86, 147-57.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed.