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Immunohistochemical analysis of paraffin-embedded mouse brain using DARPP-32 (19A3) Rabbit mAb #2306 in the presence of control peptide (left) or DARPP-32 Blocking Peptide (right).

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Product Description

This peptide is used to block DARPP-32 (19A3) Rabbit mAb #2306 reactivity in dot blot protocols.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks DARPP-32 (19A3) Rabbit mAb #2306 signal in dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).


1.  Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.

2.  Bibb, J.A. et al. (1999) Nature 402, 669-671.

3.  Fienberg, A.A. et al. (1998) Science 281, 838-842.


Entrez-Gene Id 84152
Swiss-Prot Acc. Q9UD71


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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DARPP-32 Blocking Peptide