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9183
Elk-1 Control Proteins
Experimental Controls
Protein Control Kit

Elk-1 Control Proteins #9183

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  1. WB
Western Blotting Image 1: Elk-1 Control Proteins

Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody #9186 (upper) or control Elk-1 Antibody #9182 (lower).

To Purchase # 9183S
Product # Size Price
9183S
150 µl  (10 western blots) $ 117

Product Usage Information

Boil for 3 minutes prior to use. Load 15 µl of phosphorylated and nonphosphorylated Elk1 Control Proteins.

Storage

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

Product Description

Nonphosphorylated Elk-1 Control Proteins: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer.

Phosphorylated Elk-1 Control Proteins: Bacterially expressed Elk1 fusion protein phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer.

Background

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).

Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.

  1. Marais, R. et al. (1993) Cell 73, 381-93.
  2. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
  3. Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
  4. Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
  5. Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

Pathways & Proteins

Explore pathways + proteins related to this product.

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For Research Use Only. Not For Use In Diagnostic Procedures.
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