MW (kDa) | 41, 45 |
Product Information
MW (kDa) | 41, 45 |
Activity | Elk 1 Fusion Protein at a concentration of 2 µg/20 µ| reaction was phosphorylated using p42 MAP kinase (Erk1) in an in vitro kinase assay with 1X Kinase Buffer #9802 and 200 µM ATP #9804. After a 30-minute assay at 30°, phosphorylation was detected by Western blot with Phospho-Elk-1 (Ser383) Antibody #9181. |
Molecular Formula | Apparent Molecular Weight: 41 kDa |
Elk 1 Fusion Protein, at a concentration of 2 µg/20 µl reaction, can be phosphorylated using 50 units of p42 MAP kinase (Erk2) in an in vitro kinase assay with 1X Kinase Buffer #9802 and 200 µM ATP #9804. After a 30-minute assay at 30ºC, phosphorylation can be detected by Western blot with Phospho-Elk-1 (Ser383) Antibody #9181.
The purified fusion protein was identified by SDS-PAGE and Western blot analysis, using Elk-1 Antibody #9182, to be greater than 95% pure Elk-1 .
Cloned from a human cDNA library (7) and overexpressed in E. coli.d in E. coli.
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
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