Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed

Important Shipping Information

Due to a water main break, our U.S. warehouse is closed for the remainder of the day, Thursday, July 28. Orders placed today will ship tomorrow, Friday, July 29. We apologize for the inconvenience.

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide (right).

Learn more about how we get our images
Image

Product Description

This peptide is used to block Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 signal in immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).


1.  Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.

2.  Peng, C.Y. et al. (1997) Science 277, 1501-1505.

3.  Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.

4.  Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.

5.  Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.


Entrez-Gene Id 995
Swiss-Prot Acc. P30307


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

1190
Phospho-cdc25C (Ser216) Blocking Peptide