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1255
Phospho-Chk2 (Thr68) Blocking Peptide
Experimental Controls
Blocking Peptide

Phospho-Chk2 (Thr68) Blocking Peptide #1255

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Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Storage

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Product Description

This peptide is used to block Phospho-Chk2 (Thr68) (80F5) Rabbit mAb #2584 reactivity.

Quality Control

The quality of this peptide was evaluated by reverse-phase HPLC and by mass spectrometry. The peptide blocks Phospho-Chk2 (Thr68) (80F5) Rabbit mAb #2584 signal completely in dot blot.

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

Pathways

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Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow two-four weeks for your product to be processed.