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1597
Phospho-PLK (Ser137) Biotinylated Peptide
Experimental Controls
Biotinylated Peptide

Phospho-PLK (Ser137) Biotinylated Peptide #1597

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Product Description

This biotinylated peptide contains the residues surrounding Ser137 of PLK. The serine residue in the peptide has been chemically phosphorylated in the course of peptide synthesis. This peptide was generated for use as a substrate in heterogeneous or homogeneous kinase assays.
MW (kDa) 2025
Molecular Formula 2025 daltons

Product Usage Information

This phosphorylated peptide can be detected with the Phospho-PLK (Ser137) Antibody #5070. A sample kinase assay protocol is attached.

Storage

Supplied in 0.0001% DMSO. Store at -20°C.

Quality Control

The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.

Background

At least four distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK (1). PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133, causing translocation of these proteins from the cytoplasm to the nucleus (2-5). PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to inactivate Myt1, one of the kinases known to phosphorylate cdc2 at Thr14/Tyr15 (6). Polo-like kinases also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres (7). Mitotic exit requires activation of the anaphase promoting complex (APC) (8), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A, and cdc20 (9). PLK1 phosphorylation of the APC subunits Apc1, cdc16, and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated (10,11).

Substitution of Thr210 with Asp has been reported to elevate PLK1 kinase activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest (12). In addition, while DNA damage has been found to inhibit PLK1 kinase activity, the Thr210Asp mutant is resistant to this inhibition (13). PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; DNA damage prevents phosphorylation at these sites (14).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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