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1054
PP2A A Subunit Blocking Peptide
Experimental Controls
Blocking Peptide

PP2A A Subunit Blocking Peptide #1054

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Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot and Western immunoblotting protocols.

Storage

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Product Description

This peptide is used to block PP2A A Subunit (81G5) Rabbit mAb #2041 reactivity in dot blots or Western immunoblotting.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PP2A A Subunit (81G5) Rabbit mAb #2041 signal in dot blot.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).

  1. Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39.
  2. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35.
  3. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91.
  4. Chen, J. et al. (1992) Science 257, 1261-4.
  5. Turowski, P. et al. (1995) J Cell Biol 129, 397-410.
  6. Lee, J. et al. (1996) Proc Natl Acad Sci U S A 93, 6043-7.
  7. Tolstykh, T. et al. (2000) EMBO J 19, 5682-91.
  8. Yu, X.X. et al. (2001) Mol Biol Cell 12, 185-99.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed.