Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting and dot blot protocols.
For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room
temperature for 30 minutes before allowing to react with the blot.
Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. S1% DMSO. tore at –20°C.
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody # 2217 signal completely in both dot blot and Western blotting.
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