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SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls
Experimental Controls

SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104

Western Blotting Image 1

Western blot analysis of extracts from Jurkat cells, untreated or treated with etoposide, using Cleaved Caspase-3 (Asp 175) (5A1) Rabbit mAb #9664 (upper) or Caspase-3 (8G10) Rabbit mAb #9665 (lower). This assay serves as a control for the efficacy of the etoposide treatment.

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IHC-P (paraffin) Image 2

Immunohistochemical anaysis of paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) Antibody #9661.

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Each control slide contains formalin fixed, paraffin-embedded Jurkat cells, both untreated and treated with etoposide, that serve as a control for cleaved caspase-3 (Asp 175) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the etoposide treatment.

To be used with antibodies: 9664, 9661, 9662, 2035, 9541.

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
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