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55861
SignalSlide® HeLa -/+ IFNa IHC Controls
Experimental Controls
IHC Control Slides

SignalSlide® HeLa -/+ IFNa IHC Controls #55861

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SignalSlide® HeLa -/+ IFNa IHC Controls: Image 1

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or IFNa-treated (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (top) and Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 (bottom).

To Purchase # 55861S
Product # Size Price
55861S
1 Pack  (5 slides) $ 144

Important Ordering Details

Custom Ordering Details: Sections are cut freshly upon ordering. Please allow up to three business days for your product to be processed.

Product Description

Each control slide contains formalin fixed, paraffin-embedded HeLa cells, untreated, treated with Human Interferon-α1 (hIFN-α1) #8927 that serve as a control for Phospho-Stat1 (Tyr701) and Phospho-Stat3 (Tyr705) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the hIFN-α1 treatment.

Background

Stat proteins serve as transcription factors in growth and survival pathways stimulated by growth factor and cytokine activation of receptor proteins. Receptor activation promotes tyrosine phosphorylation of Stat proteins, resulting in Stat dimerization and translocation to the nucleus where they regulate expression of numerous proteins that control cell growth, survival, differentiation and pathogen resistance (1). Stat1 is essential in IFN-α and IFN-γ stimulated pathways and is abnormally activated in many tumors (2,3). Both Stat1α (91 kDa) and Stat1β (84 kDa) isoforms are activated by IFN-α but only Stat1α responds to IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Transcription factor Stat3 possesses oncogenic potential and anti-apoptotic activities; a number of human tumors display constitutively activated Stat3 (5,6). Activation of Stat3 follows phosphorylation at Tyr705, resulting in dimerization, nuclear translocation and DNA binding (7). Expression of Stat3α (86 kDa) and Stat3β (79 kDa) isoforms correlates with cell type, ligand and cell maturation stage (8).

  1. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
  2. Bromberg, J. (2002) J Clin Invest 109, 1139-42.
  3. Frank, D.A. (1999) Mol. Med. 5, 432-456.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  6. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  7. Darnell, J.E. (1997) Science 277, 1630-5.
  8. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Pathways & Proteins

Explore pathways + proteins related to this product.

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