Buy 3 and get the 4th FREE!* | Learn More >>
1057
Thioredoxin 1 Blocking Peptide
Experimental Controls

Thioredoxin 1 Blocking Peptide #1057

Reviews ()
Citations (0)

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Storage:

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Product Description

This peptide is used to block Thioredoxin 1 (C63C6) Rabbit mAb #2429 reactivity in dot blot protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Thioredoxin 1 (C63C6) Rabbit mAb #2429 signal in dot blot.

Background

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress, and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging, and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, because it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).

  1. Watson, W.H. et al. (2004) Toxicol Sci 78, 3-14.
  2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol Sci 26, 398-404.
  3. Saitoh, M. et al. (1998) EMBO J 17, 2596-606.
  4. Callister, M.E. et al. (2007) Intensive Care Med 33, 364-7.
  5. Callister, M.E. et al. (2006) Thorax 61, 521-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed.