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|2904S||150 µl (10 western blots)||$112.00.0|
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VEGF Receptor 2 Control Proteins #2904
Gallery: VEGF Receptor 2 Control Proteins #2904
Nonphosporylated VEGFR2 Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells treated with λ phosphatase. Supplied in SDS Sample Buffer. Store at -20ºC.
Phosphorylated VEGFR2 Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells. Supplied in SDS Sample Buffer. Store at -20ºC.
As controls, we recommend using 15 µl (20 ng) of phosphorylated and nonphosphorylated VEGFR2 control proteins. Boil sample before use.Storage: Supplied in SDS Sample Buffer: 62.5 mM Tris-HCL (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C.
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
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