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a result of the USDA settlement that was
made public May 19th.

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REACTIVITY SENSITIVITY MW (kDa) SOURCE
49 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

DNMT3L Antibody (Mouse Specific) recognizes endogenous levels of total DNMT3L protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro375 of mouse DNMT3L protein. Antibodies are purified by protein A and peptide affinity chromatography.

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation.

DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1,2). These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3). DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain (4-7). The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA (4,5). The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6,7).


1.  Hermann, A. et al. (2004) Cell. Mol. Life Sci. 61, 2571-2587.

2.  Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell. Mol. Biol. Lett. 10, 631-647.

3.  Jia, D. et al. (2007) Nature 449, 248-51.

4.  Holz-Schietinger, C. and Reich, N.O. (2010) J Biol Chem 285, 29091-100.

5.  Suetake, I. et al. (2004) J Biol Chem 279, 27816-23.

6.  Ooi, S.K. et al. (2007) Nature 448, 714-7.

7.  Otani, J. et al. (2009) EMBO Rep 10, 1235-41.


Entrez-Gene Id 54427
Swiss-Prot Acc. Q9CWR8


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

12309
DNMT3L Antibody (Mouse Specific)