| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb | 12127 | 40 µl | 220 (ALK), 80 (NPM-ALK) kDa | Rabbit IgG |
| Phospho-ALK (Tyr1096) (D96H9) Rabbit mAb | 6962 | 40 µl | 80 (NPM-ALK) 220 (ALK) kDa | Rabbit IgG |
| Phospho-ALK (Tyr1278) (D59G10) Rabbit mAb | 6941 | 40 µl | 80 (NPM-ALK); 220 (ALK) kDa | Rabbit IgG |
| Phospho-ALK (Tyr1278/1282/1283) Antibody | 3983 | 40 µl | 80 (NPM-ALK), 220 (ALK) kDa | Rabbit |
| Phospho-ALK (Tyr1282/1283) (D39B2) Rabbit mAb | 9687 | 40 µl | 80 (NPM-ALK), 220 (ALK) kDa | Rabbit IgG |
| Phospho-ALK (Tyr1586) (3B4) Rabbit mAb | 3348 | 40 µl | 80 (NPM-ALK) 220 (ALK) kDa | Rabbit IgG |
| Phospho-ALK (Tyr1604) Antibody | 3341 | 40 µl | 80 (NPM-ALK) 220 (ALK) kDa | Rabbit |
| ALK (C26G7) Rabbit mAb | 3333 | 40 µl | 80 (NPM-ALK), 220 (ALK) kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The ALK Antibody Sampler Kit provides an economical means of detecting total ALK as well as ALK phosphorylated at various residues. The kit includes enough antibody to perform four western blot experiments with each primary antibody.
Storage
Background
Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5).
A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).
- Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.
- Iwahara, T. et al. (1997) Oncogene 14, 439-49.
- Morris, S.W. et al. (1997) Oncogene 14, 2175-88.
- Morris, S.W. et al. (1994) Science 263, 1281-4.
- Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.
- Soda, M. et al. (2007) Nature 448, 561-6.
Background References
Trademarks and Patents
Limited Uses
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