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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Complexin-1/2 Antibody recognizes endogenous levels of total complexin-1 and complexin-2 protein.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human complexin-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses.

1.  Chen, X. et al. (2002) Neuron 33, 397-409.

2.  McMahon, H.T. et al. (1995) Cell 83, 111-9.

3.  Reim, K. et al. (2005) J Cell Biol 169, 669-80.

4.  Reim, K. et al. (2001) Cell 104, 71-81.

5.  Huntwork, S. and Littleton, J.T. (2007) Nat Neurosci 10, 1235-7.

6.  Maximov, A. et al. (2009) Science 323, 516-21.

7.  Xue, M. et al. (2008) Proc Natl Acad Sci U S A 105, 7875-80.

Entrez-Gene Id 10815 , 10814
Swiss-Prot Acc. O14810 , Q6PUV4

For Research Use Only. Not For Use In Diagnostic Procedures.
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Complexin-1/2 Antibody