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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TIA-1 Antibody recognizes endogenous levels of total TIA-1 protein. A band of unknown origin is detected at 100 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIA-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

T-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, COX-2, cytochrome c, GADD45α, and HIF-1α (8-13).

1.  Tian, Q. et al. (1991) Cell 67, 629-39.

2.  Kawakami, A. et al. (1992) Proc Natl Acad Sci U S A 89, 8681-5.

3.  Izquierdo, J.M. and Valcárcel, J. (2007) J Biol Chem 282, 19410-7.

4.  Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.

5.  Kedersha, N.L. et al. (1999) J Cell Biol 147, 1431-42.

6.  Gilks, N. et al. (2004) Mol Biol Cell 15, 5383-98.

7.  Eisinger-Mathason, T.S. et al. (2008) Mol Cell 31, 722-36.

8.  Gottschald, O.R. et al. (2010) J Mol Cell Biol 2, 345-56.

9.  Gueydan, C. et al. (1999) J Biol Chem 274, 2322-6.

10.  Piecyk, M. et al. (2000) EMBO J 19, 4154-63.

11.  Kawai, T. et al. (2006) Mol Cell Biol 26, 3295-307.

12.  Dixon, D.A. et al. (2003) J Exp Med 198, 475-81.

13.  Lal, A. et al. (2006) Mol Cell 22, 117-28.

Entrez-Gene Id 7072
Swiss-Prot Acc. P31483

For Research Use Only. Not For Use In Diagnostic Procedures.
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TIA-1 Antibody