Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody.
Isoform specificity of 14-3-3γ (D15B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each)were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 γ (D15B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).
Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb.
Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.
Isoform specificity of 14-3-3 η (D23B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each) were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 η (D23B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.
Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.
|14-3-3 β/α Antibody 9636||20 µl||
||H Mk M R||28||Rabbit|
|14-3-3 γ (D15B7) Rabbit mAb 5522||20 µl||
||H Mk M Pg R||27||Rabbit IgG|
|14-3-3 ζ/δ (D7H5) Rabbit mAb 7413||20 µl||
||H Mk M Pg R||28||Rabbit IgG|
|14-3-3 ε Antibody 9635||20 µl||
||H Mk M R||28||Rabbit|
|14-3-3 τ Antibody 9638||20 µl||
||H Mk M R||28||Rabbit|
|14-3-3 η (D23B7) Rabbit mAb 5521||20 µl||
||B H Mk M Pg R||27||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
The 14-3-3 Family Antibody Sampler Kit provides an economical means to investigate the expression of various 14-3-3 isoforms within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.
Each antibody in the 14-3-3 Family Antibody Sampler Kit detects endogenous levels of its respective target.
Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequences of human 14-3-3 ß/α, 14-3-3 ε and 14-3-3 τ proteins. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein, Leu37 of human 14-3-3 η protein and Ile79 of human 14-3-3 γ protein.
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
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