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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

AUP1 Antibody recognizes endogenous levels of total AUP1 protein.

Species predicted to react based on 100% sequence homology: Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln320 of human AUP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Ancient ubiquitous protein 1 (AUP1) is a component of the ER-associated protein degradation (ERAD) machinery responsible for the ubiquitin-mediated degradation of misfolded proteins (1). AUP1 protein contains four conserved domains, with a long, amino-terminal stretch of hydrophobic amino acids followed by an acyltransferase domain (2). Amino-terminal protein sequences direct localization of AUP1 to both the ER and to cytosolic lipid droplets (3). The AUP1 CUE domain binds ubiquitin (4), while the G2BR domain allows for association between AUP1 and E2 conjugating enzyme UBE2G2 (5,6). The presence of these binding domains suggests a central role for AUP1 in the ubiquitination-mediated protein degradation (2). Research studies indicate that AUP1 recruits UBE2G2 to cytosolic lipid droplets, ER-derived organelles that are sites for storage and hydrolysis of neutral lipids. Inhibition of AUP1 protein function results in decreased ubiquitin-mediated degradation of several proteins, including the cholesterol biosynthetic enzyme HMG-CoA-reductase and the cholesterol synthesis regulator INSIG1 (6).

1.  Mueller, B. et al. (2008) Proc Natl Acad Sci U S A 105, 12325-30.

2.  Spandl, J. et al. (2011) J Biol Chem 286, 5599-606.

3.  Stevanovic, A. and Thiele, C. (2013) J Lipid Res 54, 503-13.

4.  Lohmann, D. et al. (2013) PLoS One 8, e72453.

5.  Klemm, E.J. et al. (2011) J Biol Chem 286, 37602-14.

6.  Jo, Y. et al. (2013) Mol Biol Cell 24, 169-83.

Entrez-Gene Id 550
Swiss-Prot Acc. Q9Y679

For Research Use Only. Not For Use In Diagnostic Procedures.
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AUP1 Antibody