WB
M
Transfected Only
110
Rabbit
#O00206
7099
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys549 within the extracellular region of mouse and rat TLR4 protein. Antibodies were purified by peptide affinity chromatography.
Background
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.
TLR4 functions in association with MD-2 in the recognition and initiation of immune responses elicited by lipopolysaccharide (LPS) of Gram-negative bacteria (4-8). TLR4 triggers the activation of NF-κB, IRF-3, and MAPK pathways leading to the production of inflammatory cytokines (9).
- Akira, S. (2003) J Biol Chem 278, 38105-8.
- Beutler, B. (2004) Nature 430, 257-63.
- Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
- Medzhitov, R. et al. (1997) Nature 388, 394-7.
- Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
- Takeuchi, O. et al. (1999) Immunity 11, 443-51.
- Alexopoulou, L. et al. (2001) Nature 413, 732-8.
- Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
- Horng, T. et al. (2001) Nat Immunol 2, 835-41.
- Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
- Muzio, M. et al. (1997) Science 278, 1612-5.
- Wesche, H. et al. (1997) Immunity 7, 837-47.
- Suzuki, N. et al. (2002) Nature 416, 750-6.
- Irie, T. et al. (2000) FEBS Lett 467, 160-4.
- Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 588-593.
- Poltorak, A. et al. (1998) Science 282, 2085-2088.
- Chow, J.C. et al. (1999) J. Biol. Chem. 274, 10689-10692.
- Hoshino, K. et al. (1999) J. Immunol. 162, 3749-3752.
- Shimazu, R. et al. (1999) J. Exp. Med. 189, 1777-1782.
- Kawai, T. and Akira, S. (2006) Cell Death Differ. 13, 816-825.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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