Upstream / Downstream


Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee



Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


We recommend the following alternatives

W IP   IF F   H M R Mk
W IP   IF F   H
72, 74 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Numb Antibody detects endogenous levels of total Numb protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb. Antibodies are purified by protein A and peptide affinity chromatography.

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

1.  Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.

2.  Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.

3.  Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.

4.  Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.

5.  Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.

6.  McGill, M.A. and McGlade, C.J. (2003) J Biol Chem 278, 23196-203.

7.  Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.

8.  Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.

9.  Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

Entrez-Gene Id 8650
Swiss-Prot Acc. P49757

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Numb Antibody