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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Bcl-2 Antibody detects endogenous levels of total Bcl-2 alpha protein. This antibody does not cross-react with Bcl-2 beta or other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of Bcl-2 alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

1.  Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.

2.  Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.

3.  Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.

4.  Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.

5.  Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.

6.  Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.

7.  Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

Entrez-Gene Id 596
Swiss-Prot Acc. P10415

For Research Use Only. Not For Use In Diagnostic Procedures.
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Bcl-2 Antibody