WB, IP
H M
Endogenous
70-120
Rabbit IgG
#Q9BZW8
51744
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp367 of human 2B4/SLAMF4/CD244 protein.
Background
2B4, also known as signaling lymphocytic activation molecule family member 4 (SLAMF4) and cluster of differentiation 244 (CD244), is a heterophilic cell surface receptor expressed on a variety of immune cells, including natural killer (NK) cells, T cells, eosinophils, mast cells, and dendritic cells. 2B4 has been shown to have both immune stimulatory and inhibitory effects on cells (1-11). Upon engagement of its ligand CD48, 2B4 can enhance immune cell signaling, cytokine production, non-major histocompatibility complex-restricted cytotoxicity, and cell migration (1-8). Conversely, at early stages of NK cell differentiation, 2B4 may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (10). 2B4 also inhibits inflammatory responses in dendritic cells (11). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Speiser, D.E. et al. (2001) J Immunol 167, 6165-70.
- Nakajima, H. et al. (1999) Eur J Immunol 29, 1676-83.
- Valiante, N.M. and Trinchieri, G. (1993) J Exp Med 178, 1397-406.
- Munitz, A. et al. (2005) J Immunol 174, 110-8.
- Sivori, S. et al. (2000) Eur J Immunol 30, 787-93.
- El-Shazly, A.E. et al. Int J Immunopathol Pharmacol 24, 949-60.
- Chen, L. and Flies, D.B. (2013) Nat Rev Immunol 13, 227-42.
- Assarsson, E. et al. (2005) Mol Immunol 42, 419-23.
- Elishmereni, M. et al. (2014) J Invest Dermatol 134, 2928-37.
- Sivori, S. et al. (2002) Proc Natl Acad Sci U S A 99, 4526-31.
- Georgoudaki, A.M. et al. (2015) Immunol Cell Biol 93, 581-90.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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