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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

HNF4α (G162) Antibody detects endogenous levels of total HNF4α protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human HNF4α. Antibodies are purified by protein A and peptide affinity chromatography.

Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).

1.  Sladek, F.M. et al. (1990) Genes Dev. 4, 2353-2365.

2.  Yoon, J.C. et al. (2001) Nature 413, 131-138.

3.  Rhee, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4012-4017.

4.  Hayhurst, G.P. et al. (2001) Mol. Cell Biol. 21, 1393-1403.

5.  Ellard, S. and Colclough, K. (2006) Hum. Mutat. 27, 854-869.

Entrez-Gene Id 3172
Swiss-Prot Acc. P41235

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

HNF4α (G162) Antibody