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We recommend the following alternatives

W           H M R Mk
W IP   IF     H M R Hm Mk B
REACTIVITY SENSITIVITY MW (kDa) SOURCE
46, 50 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

VASP (A290) Antibody detects endogenous levels of total VASP protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding the carboxy-terminal sequence of human VASP. Antibodies are purified by peptide affinity chromatography.

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).


1.  Butt, E. et al. (1994) J Biol Chem 269, 14509-17.

2.  Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25.

3.  Oelze, M. et al. (2000) Circ Res 87, 999-1005.

4.  Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6.

5.  Ball, L.J. et al. (2000) EMBO J 19, 4903-14.

6.  Machesky, L.M. (2000) Cell 101, 685-8.

7.  Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35.


Entrez-Gene Id 7408
Swiss-Prot Acc. P50552


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

3120
VASP (A290) Antibody