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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-CaMKII (Thr286) Antibody detects endogenous levels of CaMKII only when phosphorylated at Thr286.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

1.  Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.

2.  Barria, A. et al. (1997) Science 276, 2042-5.

3.  Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63.

4.  Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

5.  Banno, Y. et al. (2008) J Neurochem 104, 1372-86.

Entrez-Gene Id 815 , 816 , 817 , 818
Swiss-Prot Acc. Q9UQM7 , Q13554 , Q13557 , Q13555

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Phospho-CaMKII (Thr286) Antibody