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REACTIVITY SENSITIVITY MW (kDa) Isotype
55 Mouse 

Product Usage Information

Not recommended for use with fixation/permeabilization protocols that contain methanol.


Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD4 (Edu-2) Mouse mAb detects endogenous levels of total CD4 protein.


Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.

Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).


1.  Zamoyska, R. (1994) Immunity 1, 243-46.


Entrez-Gene Id 920
Swiss-Prot Acc. P01730


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

3563
CD4 (Edu-2) Mouse mAb