Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Phospho-TNK1 (Tyr277) Antibody detects endogenous levels of TNK1 protein only when phosphorylated at Tyr277. This antibody may cross-react with tyrosine phosphorylated EGF receptor.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence around Tyr277 of human TNK1. Antibodies are purified by protein A and peptide affinity chromatography.
Tyrosine kinase non-receptor 1 (TNK1) is related to the Ack1 (TNK2) non-receptor kinase that binds cdc42 and inhibits GTPase activity of this cell cycle regulator. TNK1 is broadly expressed in embryogenic tissues and leukemia cell lines, but is restricted to select adult tissues (1). TNK1 is a putative 72 kDa protein comprised of an N-terminal kinase domain, a central SH3 domain and a proline-rich tail. Interaction with PLCγ in vitro indicates a possible role in phospholipid signal transduction pathways (2). Though the exact mechanism is currently unclear, active TNK1 may play a role in regulating cell death by preventing TNF-α-induced NF-κB transactivation (3).
Phosphorylation of TNK1 on Tyr277 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (4) and also reported independently in another publication using MS technology (5). Phosphorylation of TNK1 at Tyr277 was observed in select carcinoma cell lines and in tumors. A constitutively active, truncated TNK1 kinase resulting from fusion between the TNK1 and C17orf61 genes is seen in some cells (5). For additional information visit PhosphoSitePlus™, CST's modification site knowledgebase, at www.phosphosite.org.
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