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REACTIVITY SENSITIVITY MW (kDa) SOURCE
145 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-SMC1 (Ser957) Antibody detects endogenous levels of SMC1 only when phosphorylated at Ser957.


Species predicted to react based on 100% sequence homology: Xenopus, Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1. Antibodies are purified by protein A and peptide affinity chromatography.

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).


1.  Michaelis, C. et al. (1997) Cell 91, 35-45.

2.  Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.

3.  Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.

4.  Kim, S.T. et al. (2002) Genes Dev 16, 560-70.


Entrez-Gene Id 8243
Swiss-Prot Acc. Q14683


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

4801
Phospho-SMC1 (Ser957) Antibody