WB, IHC-P, IF-IC, FC-FP, FC-L
H
Endogenous
75-120
Rabbit IgG
#P08195
6520
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val509 of human 4F2hc/SLC3A2 protein.
Background
4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters such as LAT1 and LAT2 and regulates uptake of amino acids (1-5). 4F2hc is one of the earliest expressed antigens on the surface of activated human lymphocytes (6), hence it is also named CD98. 4F2hc is expressed in all cell types with the exception of platelets, and is expressed at highest levels in the tubules of the kidney and the gastrointestinal tract (7,8). It is localized at the plasma membrane when associated with LAT1 or LAT2 (9) and at the apical membrane of placenta (10). Research studies have shown that 4F2hc is highly expressed in various tumors including glioma (11), ovarian cancer (12), and astrocytomas (13), and it has been implicated in tumor progression and correlated with poor outcome in patients with pulmonary neuroendocrine tumors (14). 4F2hc is also involved in integrin trafficking through association with β1 and β4 integrins, and regulates keratinocyte adhesion and differentiation (15).
- Kanai, Y. et al. (1998) J Biol Chem 273, 23629-32.
- Mastroberardino, L. et al. (1998) Nature 395, 288-91.
- Pfeiffer, R. et al. (1999) EMBO J 18, 49-57.
- Pineda, M. et al. (1999) J Biol Chem 274, 19738-44.
- Sato, H. et al. (1999) J Biol Chem 274, 11455-8.
- Lumadue, J.A. et al. (1987) Proc Natl Acad Sci USA 84, 9204-8.
- Rossier, G. et al. (1999) J Biol Chem 274, 34948-54.
- Dave, M.H. et al. (2004) J Physiol 558, 597-610.
- Bröer, A. et al. (2001) Biochem J 355, 725-31.
- Ritchie, J.W. and Taylor, P.M. (2001) Biochem J 356, 719-25.
- Okubo, S. et al. (2010) J Neurooncol 99, 217-25.
- Kaji, M. et al. (2010) Int J Gynecol Cancer 20, 329-36.
- Nawashiro, H. et al. (2006) Int J Cancer 119, 484-92.
- Kaira, K. et al. (2011) Oncol Rep 26, 931-7.
- Lemaître, G. et al. (2011) J Dermatol Sci 61, 169-79.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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